![]() ![]() Count the cells in one set of 16 squares (1×1 mm square area the blue area). ![]() Using a microscope, focus on the grid lines of the counting area with a 5-10x objective. Enough liquid should be introduced so that the counting surface is just covered. The capillary force will drag the liquid to fill the area under the coverslip. Introduce the cell suspension (10 μL or one drop) from the edge of the coverslip with a fine tip transfer pipette. Place the coverslip over the counting surface. If the cell concentration is too high, try to do 1:10, 1:100, 1:1000 serial dilutions (see the illustration below). If the cell concentration is too low, you need to count more squares and perhaps more times to avoid the statistical errors. If the cell concentration is too high, it is hard to count cells visually under the microscope. The concentration of cells should not be too high or too low. Mixing the sample well before taking the samples. ![]()
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